The draw for Round Two of the Papa John’s Trophy will take place live on Sky Sports Football on Saturday 13 November straight after the 12pm kick off.
The draw will be hosted by David Prutton and conducted by former Newport County Manager Michael Flynn, alongside Chris Birchall.
Round Two remains split regionally into a Northern and Southern section with group winners drawn at home against a runner-up from another group.
In the Northern section, Rotherham United and Bolton Wanderers are already through to Round Two after topping their respective groups with a maximum of nine points. In the Southern section, Aston Villa U21 will be in the hat for the next round alongside MK Dons, Charlton Athletic and Leyton Orient who have also qualified for the next stage of the competition.
The final group matches take place 9-10 November with two Clubs progressing to the last 32 from each group.
Round Two ties will be played week commencing 29 November.